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KMID : 0380020170320020153
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Korean Journal of Biotechnology and Bioengineering
2017 Volume.32 No. 2 p.153 ~ p.159
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Antibacterial Activity and Probiotic Properties of Lactic Acid Bacteria from Korean Intestine Origin
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Kang Chang-Ho
Kim Yong-Gyeong
Han Seul-Hwa
Jeong Yul-Ah
Paek Nam-Soo
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Abstract
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The purpose of this study was to investigate the probiotic properties of lactic acid bacterial strains isolated from human feces. The properties were tested on the basis of guideline for probiotic selection protocol such as tolerance for acid or bile salt, autoaggregation, antibiotic resistance, and antimicrobial activity. Total 25 lactic acid bacteria were isolated from human feces, and their antibacterial activity was tested against Staphylococcus aureus, Escherichia coli, E. coli O157:H7, Vibrio parahaemolyticus, V. alginolyticus using an agar diffusion assay. Among them, 4 selected strains were identified by analysis of their 16S rRNA, as Lactobacillus rhamnosus MG316, L. acidophilus MG501, L. reuteri MG505, and L. gasseri MG570. Results show that resistance to low pH and bile salts. Also, the selected strains were resistant to bile acid up to 3% and their autoaggregation rates were as high as 60%. All strains tested were resistance to nalidixic acid and kanamycin.
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KEYWORD
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Probiotic, Lactic acid bacteria, Human feces, Antimicrobial activity
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